Comparison of the radioisotope dilution-coated charcoal method and a microbiological method (L. leichmannii) for measuring vitamin B12 in serum.
نویسندگان
چکیده
A comparison has been made of the radioisotope dilution-coated charcoal method and a microbiological assay (with L. leichmannii as test organism) for determining the concentration of vitamin B(12) in serum. A satisfactory correlation was found between the results of the two methods. Under appropriate conditions the reproducibility of the radioisotope method compared favourably with that of the microbiological method.
منابع مشابه
Interlaboratory comparison of serum vitamin B12 assay.
The results have been compared of microbiological and radioisotope dilution (RID) assay of serum vitamin B12 by participants in national interlaboratory trials in Britain. There was wide variation between the individual participants, especially marked in the L. leichmannii microbiological assay and in the RID methods, whereas excellent correlation, reproducibility, and recovery were obtained in...
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An automated method for the microbiological assay of vitamin B(12) is described. A chloramphenicol-resistant strain of Lactobacillus leichmannii is used as the test organism and this eliminates the need for sterilization or aseptic addition. Precipitation of the serum protein is avoided by dilution of the serum with a glutamic/malic acid solution before heating the preparation to free the vitam...
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عنوان ژورنال:
- Journal of clinical pathology
دوره 19 6 شماره
صفحات -
تاریخ انتشار 1966